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    Developmental Studies Hybridoma Bank type iib muscle fibers
    Systemic transplantation of young MDSPCs promotes skeletal <t>muscle</t> neovascularization, improves muscle structure, and increases mitochondrial content. (A) Representative immunohistochemical images of gastrocnemius (GS) muscles from young MDSPC (NA‐CI; n = 9) and PBS (NA‐PBS; n = 7) treated mice, labeled for CD31 (red) and dystrophin (green). (B) Quantification of vasculature density, presented as the number of vessels per muscle fiber. (C) Quantification of total vasculature area, presented as percentage of muscle area occupied by vessels. (D) Volcano plot depicting statistical significance and fold change of neovascularization pathway protein phosphorylation levels in GS muscles from NA‐CI ( n = 4) and NA‐PBS ( n = 4) mice. Proteins that have sites with significantly increased phosphorylation in NA‐CI muscles are highlighted in green. (E) Representative images of GS muscles stained with Sirius red (collagen, red) and Fast Green (muscle, green) at 2 months post‐intraperitoneal (IP) transplantation. (F) Quantification of collagen content as a percentage of total tissue area. (G) Representative images of dystrophin‐labeled GS muscles (green) from NA‐CI and NA‐PBS mice. (H) Violin plot of muscle fiber cross‐sectional area (CSA), with lines indicating median and interquartile ranges (25th and 75th percentiles). (I) Frequency distribution of muscle fiber CSA binned in 100 μm 2 intervals. (J) Citrate synthase activity measured in quadriceps muscles of NA‐CI ( n = 9) and NA‐PBS ( n = 7) mice. (K) Representative images of GS muscles labeled for <t>type</t> I (blue), type IIa (green), and type <t>IIb</t> (red) muscle <t>fibers</t> at 2 months post‐IP transplantation. (L) Stacked bar plot of fiber type composition in GS muscles of NA‐CI ( n = 7) and NA‐PBS ( n = 7) mice. Data (B, C, F, I, J, L) are presented as mean ± SEM. ** p ≤ 0.01, *** p ≤ 0.001, and § p ≤ 0.0001 using one‐tailed unpaired Student's t ‐test. (H) § p ≤ 0.0001 by two‐tailed Kolmogorov–Smirnov test. Scale bars are 100 μm (A, G, K) and 500 μm (E).
    Type Iib Muscle Fibers, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+iib+muscle+fibers/anti-Myosin+heavy+chain+Type+IIB/pmc12895213-217-28-32
    Average 96 stars, based on 808 article reviews
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    Images

    1) Product Images from "Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health"

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health

    Journal: Aging Cell

    doi: 10.1111/acel.70408

    Systemic transplantation of young MDSPCs promotes skeletal muscle neovascularization, improves muscle structure, and increases mitochondrial content. (A) Representative immunohistochemical images of gastrocnemius (GS) muscles from young MDSPC (NA‐CI; n = 9) and PBS (NA‐PBS; n = 7) treated mice, labeled for CD31 (red) and dystrophin (green). (B) Quantification of vasculature density, presented as the number of vessels per muscle fiber. (C) Quantification of total vasculature area, presented as percentage of muscle area occupied by vessels. (D) Volcano plot depicting statistical significance and fold change of neovascularization pathway protein phosphorylation levels in GS muscles from NA‐CI ( n = 4) and NA‐PBS ( n = 4) mice. Proteins that have sites with significantly increased phosphorylation in NA‐CI muscles are highlighted in green. (E) Representative images of GS muscles stained with Sirius red (collagen, red) and Fast Green (muscle, green) at 2 months post‐intraperitoneal (IP) transplantation. (F) Quantification of collagen content as a percentage of total tissue area. (G) Representative images of dystrophin‐labeled GS muscles (green) from NA‐CI and NA‐PBS mice. (H) Violin plot of muscle fiber cross‐sectional area (CSA), with lines indicating median and interquartile ranges (25th and 75th percentiles). (I) Frequency distribution of muscle fiber CSA binned in 100 μm 2 intervals. (J) Citrate synthase activity measured in quadriceps muscles of NA‐CI ( n = 9) and NA‐PBS ( n = 7) mice. (K) Representative images of GS muscles labeled for type I (blue), type IIa (green), and type IIb (red) muscle fibers at 2 months post‐IP transplantation. (L) Stacked bar plot of fiber type composition in GS muscles of NA‐CI ( n = 7) and NA‐PBS ( n = 7) mice. Data (B, C, F, I, J, L) are presented as mean ± SEM. ** p ≤ 0.01, *** p ≤ 0.001, and § p ≤ 0.0001 using one‐tailed unpaired Student's t ‐test. (H) § p ≤ 0.0001 by two‐tailed Kolmogorov–Smirnov test. Scale bars are 100 μm (A, G, K) and 500 μm (E).
    Figure Legend Snippet: Systemic transplantation of young MDSPCs promotes skeletal muscle neovascularization, improves muscle structure, and increases mitochondrial content. (A) Representative immunohistochemical images of gastrocnemius (GS) muscles from young MDSPC (NA‐CI; n = 9) and PBS (NA‐PBS; n = 7) treated mice, labeled for CD31 (red) and dystrophin (green). (B) Quantification of vasculature density, presented as the number of vessels per muscle fiber. (C) Quantification of total vasculature area, presented as percentage of muscle area occupied by vessels. (D) Volcano plot depicting statistical significance and fold change of neovascularization pathway protein phosphorylation levels in GS muscles from NA‐CI ( n = 4) and NA‐PBS ( n = 4) mice. Proteins that have sites with significantly increased phosphorylation in NA‐CI muscles are highlighted in green. (E) Representative images of GS muscles stained with Sirius red (collagen, red) and Fast Green (muscle, green) at 2 months post‐intraperitoneal (IP) transplantation. (F) Quantification of collagen content as a percentage of total tissue area. (G) Representative images of dystrophin‐labeled GS muscles (green) from NA‐CI and NA‐PBS mice. (H) Violin plot of muscle fiber cross‐sectional area (CSA), with lines indicating median and interquartile ranges (25th and 75th percentiles). (I) Frequency distribution of muscle fiber CSA binned in 100 μm 2 intervals. (J) Citrate synthase activity measured in quadriceps muscles of NA‐CI ( n = 9) and NA‐PBS ( n = 7) mice. (K) Representative images of GS muscles labeled for type I (blue), type IIa (green), and type IIb (red) muscle fibers at 2 months post‐IP transplantation. (L) Stacked bar plot of fiber type composition in GS muscles of NA‐CI ( n = 7) and NA‐PBS ( n = 7) mice. Data (B, C, F, I, J, L) are presented as mean ± SEM. ** p ≤ 0.01, *** p ≤ 0.001, and § p ≤ 0.0001 using one‐tailed unpaired Student's t ‐test. (H) § p ≤ 0.0001 by two‐tailed Kolmogorov–Smirnov test. Scale bars are 100 μm (A, G, K) and 500 μm (E).

    Techniques Used: Transplantation Assay, Immunohistochemical staining, Muscles, Labeling, Phospho-proteomics, Staining, Activity Assay, One-tailed Test, Two Tailed Test

    Related Articles

    Immunofluorescence:

    Article Title: Oxidative stress causes muscle structural alterations via p38 MAPK signaling in COPD mouse model.
    Article Snippet: Introduction Sarcopenia is a complication of Chronic Obstructive Pulmonary Disease (COPD) that negatively affects physical activity and quality of life.. However, the underlying mechanism by which COPD affects skeletal muscles remains to be elucidated.. Therefore, we investigated the association between oxidative stress and structural alterations in muscles in elastase-induced emphysema mouse models.

    Article Title: Systemic bone loss, impaired osteogenic activity and type I muscle fiber atrophy in mice with elastase-induced pulmonary emphysema: Establishment of a COPD-related osteoporosis mouse model.
    Article Snippet: Please cite this article as: Manabu Tsukamoto, Toshiharu Mori, Ke-Yong Wang, Yasuaki Okada, Hokuto Fukuda, Keisuke Naito, Yoshiaki Yamanaka, Ken Sabanai, Eiichiro Nakamura, Kazuhiro Yatera, Akinori Sakai , Systemic bone loss, impaired osteogenic activity and type I muscle fiber atrophy in mice with elastase-induced pulmonary emphysema: Establishment of a COPD-related osteoporosis mouse model. Bon (2018), doi:10.1016/j.bone.2018.10.017

    Staining:

    Article Title: Oxidative stress causes muscle structural alterations via p38 MAPK signaling in COPD mouse model.
    Article Snippet: Introduction Sarcopenia is a complication of Chronic Obstructive Pulmonary Disease (COPD) that negatively affects physical activity and quality of life.. However, the underlying mechanism by which COPD affects skeletal muscles remains to be elucidated.. Therefore, we investigated the association between oxidative stress and structural alterations in muscles in elastase-induced emphysema mouse models.

    Article Title: Systemic bone loss, impaired osteogenic activity and type I muscle fiber atrophy in mice with elastase-induced pulmonary emphysema: Establishment of a COPD-related osteoporosis mouse model.
    Article Snippet: Please cite this article as: Manabu Tsukamoto, Toshiharu Mori, Ke-Yong Wang, Yasuaki Okada, Hokuto Fukuda, Keisuke Naito, Yoshiaki Yamanaka, Ken Sabanai, Eiichiro Nakamura, Kazuhiro Yatera, Akinori Sakai , Systemic bone loss, impaired osteogenic activity and type I muscle fiber atrophy in mice with elastase-induced pulmonary emphysema: Establishment of a COPD-related osteoporosis mouse model. Bon (2018), doi:10.1016/j.bone.2018.10.017

    Immunohistochemistry:

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health
    Article Snippet: .. Muscle fiber cross‐sectional area was assessed by immunohistochemically (IHC) labeling dystrophin (Abcam, ab15277, 1:300), type I muscle fibers (DSHB, BA‐F8, 1:50), type IIa muscle fibers (DSHB, SC‐71, 1:600), type IIb muscle fibers (DSHB, BF‐F3, 1:100), and type IIx muscle fibers (DSHB, 6H1, 1:50), following established protocols (Vella et al. ; Lavasani et al. , ; Bloemberg and Quadrilatero ). .. Dystrophin images were acquired using a Leica DM6000 automated upright microscope and muscle fiber type images were acquired using an Olympus Slideview VS200, both using a 20× plan APO objective.

    Labeling:

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health
    Article Snippet: .. Muscle fiber cross‐sectional area was assessed by immunohistochemically (IHC) labeling dystrophin (Abcam, ab15277, 1:300), type I muscle fibers (DSHB, BA‐F8, 1:50), type IIa muscle fibers (DSHB, SC‐71, 1:600), type IIb muscle fibers (DSHB, BF‐F3, 1:100), and type IIx muscle fibers (DSHB, 6H1, 1:50), following established protocols (Vella et al. ; Lavasani et al. , ; Bloemberg and Quadrilatero ). .. Dystrophin images were acquired using a Leica DM6000 automated upright microscope and muscle fiber type images were acquired using an Olympus Slideview VS200, both using a 20× plan APO objective.



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    Image Search Results


    Systemic transplantation of young MDSPCs promotes skeletal muscle neovascularization, improves muscle structure, and increases mitochondrial content. (A) Representative immunohistochemical images of gastrocnemius (GS) muscles from young MDSPC (NA‐CI; n = 9) and PBS (NA‐PBS; n = 7) treated mice, labeled for CD31 (red) and dystrophin (green). (B) Quantification of vasculature density, presented as the number of vessels per muscle fiber. (C) Quantification of total vasculature area, presented as percentage of muscle area occupied by vessels. (D) Volcano plot depicting statistical significance and fold change of neovascularization pathway protein phosphorylation levels in GS muscles from NA‐CI ( n = 4) and NA‐PBS ( n = 4) mice. Proteins that have sites with significantly increased phosphorylation in NA‐CI muscles are highlighted in green. (E) Representative images of GS muscles stained with Sirius red (collagen, red) and Fast Green (muscle, green) at 2 months post‐intraperitoneal (IP) transplantation. (F) Quantification of collagen content as a percentage of total tissue area. (G) Representative images of dystrophin‐labeled GS muscles (green) from NA‐CI and NA‐PBS mice. (H) Violin plot of muscle fiber cross‐sectional area (CSA), with lines indicating median and interquartile ranges (25th and 75th percentiles). (I) Frequency distribution of muscle fiber CSA binned in 100 μm 2 intervals. (J) Citrate synthase activity measured in quadriceps muscles of NA‐CI ( n = 9) and NA‐PBS ( n = 7) mice. (K) Representative images of GS muscles labeled for type I (blue), type IIa (green), and type IIb (red) muscle fibers at 2 months post‐IP transplantation. (L) Stacked bar plot of fiber type composition in GS muscles of NA‐CI ( n = 7) and NA‐PBS ( n = 7) mice. Data (B, C, F, I, J, L) are presented as mean ± SEM. ** p ≤ 0.01, *** p ≤ 0.001, and § p ≤ 0.0001 using one‐tailed unpaired Student's t ‐test. (H) § p ≤ 0.0001 by two‐tailed Kolmogorov–Smirnov test. Scale bars are 100 μm (A, G, K) and 500 μm (E).

    Journal: Aging Cell

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health

    doi: 10.1111/acel.70408

    Figure Lengend Snippet: Systemic transplantation of young MDSPCs promotes skeletal muscle neovascularization, improves muscle structure, and increases mitochondrial content. (A) Representative immunohistochemical images of gastrocnemius (GS) muscles from young MDSPC (NA‐CI; n = 9) and PBS (NA‐PBS; n = 7) treated mice, labeled for CD31 (red) and dystrophin (green). (B) Quantification of vasculature density, presented as the number of vessels per muscle fiber. (C) Quantification of total vasculature area, presented as percentage of muscle area occupied by vessels. (D) Volcano plot depicting statistical significance and fold change of neovascularization pathway protein phosphorylation levels in GS muscles from NA‐CI ( n = 4) and NA‐PBS ( n = 4) mice. Proteins that have sites with significantly increased phosphorylation in NA‐CI muscles are highlighted in green. (E) Representative images of GS muscles stained with Sirius red (collagen, red) and Fast Green (muscle, green) at 2 months post‐intraperitoneal (IP) transplantation. (F) Quantification of collagen content as a percentage of total tissue area. (G) Representative images of dystrophin‐labeled GS muscles (green) from NA‐CI and NA‐PBS mice. (H) Violin plot of muscle fiber cross‐sectional area (CSA), with lines indicating median and interquartile ranges (25th and 75th percentiles). (I) Frequency distribution of muscle fiber CSA binned in 100 μm 2 intervals. (J) Citrate synthase activity measured in quadriceps muscles of NA‐CI ( n = 9) and NA‐PBS ( n = 7) mice. (K) Representative images of GS muscles labeled for type I (blue), type IIa (green), and type IIb (red) muscle fibers at 2 months post‐IP transplantation. (L) Stacked bar plot of fiber type composition in GS muscles of NA‐CI ( n = 7) and NA‐PBS ( n = 7) mice. Data (B, C, F, I, J, L) are presented as mean ± SEM. ** p ≤ 0.01, *** p ≤ 0.001, and § p ≤ 0.0001 using one‐tailed unpaired Student's t ‐test. (H) § p ≤ 0.0001 by two‐tailed Kolmogorov–Smirnov test. Scale bars are 100 μm (A, G, K) and 500 μm (E).

    Article Snippet: Muscle fiber cross‐sectional area was assessed by immunohistochemically (IHC) labeling dystrophin (Abcam, ab15277, 1:300), type I muscle fibers (DSHB, BA‐F8, 1:50), type IIa muscle fibers (DSHB, SC‐71, 1:600), type IIb muscle fibers (DSHB, BF‐F3, 1:100), and type IIx muscle fibers (DSHB, 6H1, 1:50), following established protocols (Vella et al. ; Lavasani et al. , ; Bloemberg and Quadrilatero ).

    Techniques: Transplantation Assay, Immunohistochemical staining, Muscles, Labeling, Phospho-proteomics, Staining, Activity Assay, One-tailed Test, Two Tailed Test